2-Dodecyl-6-methoxycyclohexa-2, 5-dien-1,4-dione alleviates liver fibrosis and improves intestinal flora and bile acid metabolism

2-Dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD), a bioactive compound of star fruit horn root, has shown therapeutic potential by regulating the TGF-β1 pathway and regulating bile acids.In this study, a liver fibrosis model was established in Kunming mice (KM) induced by carbon tetrachloride (CCL4), and DMDD (50 mg/kg) was administered intragastrically. Biochemical indices and immunohistochemical staining were used to evaluate the effect of DMDD on liver fibrosis. The Illumina sequencing platform was used to detect intestinal flora and liver transcriptome information in mouse feces, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology was used to detect bile acid content changes in mouse feces.“2-Dodecyl-6-methoxycyclohexa-2, 5-Dien-1, 4-Dione alleviates liver fibrosis and improves intestinal flora and bile acid metabolism "Supplementary material to the manuscript.

2-十二烷基-6-甲氧基环己-2,5-二烯-1,4-二酮（DMDD）作为杨桃根的生物活性成分，可通过调控转化生长因子-β1（TGF-β1）通路与胆汁酸代谢展现治疗潜力。本研究采用四氯化碳（CCL4）诱导昆明小鼠（KM）构建肝纤维化模型，以50 mg/kg的剂量对小鼠灌胃给予DMDD。通过生化指标与免疫组织化学染色评估DMDD对肝纤维化的干预效果；利用Illumina测序平台检测小鼠粪便中的肠道菌群与肝脏转录组信息，并借助高效液相色谱-串联质谱（HPLC-MS/MS）技术测定小鼠粪便的胆汁酸含量变化。
本研究相关稿件补充材料：2-十二烷基-6-甲氧基环己-2,5-二烯-1,4-二酮可缓解肝纤维化并改善肠道菌群与胆汁酸代谢。