Mirounga angustirostris blubber transcriptome (single ACTH challenge)

Juvenile northern elephant seal (Mirounga angustirostris) blubber transcriptome assemblies and annotation. Blubber biopsies were collected during an acute stress challenge induced by administration of a single 30-U dose of adrenocorticotropic hormone (ACTH; Oct-Nov 2014). Blubber samples were collected immediately prior to ("baseline") and 2 and 24 hours following ACTH administration ("acute response" and "recovery", respectively). Mean RNA integrity value for samples was 8.04. Library preparation and Illumina HiSeq 2500 sequencing were conducted at UC Davis DNA Technologies Core Facility using standard protocols. Four baseline, four acute response, and two recovery samples were 100-bp paired-end sequenced on a single Illumina lane, generating mean 32 million reads per sample (SRA Accession: SRP045540). Reads were quality trimmed and abundance normalized using one round of diginorm (khmer v0.8.4; coverage: 20X, k-mer: 20). The raw assembly was produced de novo using Trinity v2.0.6 with default settings. The overall TransRate (v1.0.1) quality score for the assembly was 0.42. The assembly contained 80% of highly conserved vertebrate orthologs (BUSCOs). The filtered assembly was produced using TransDecoder v2.0.1. The annotation report was produced using Trinotate v3.0.0. (SwissProt database downloaded on 3/15/16).

幼年北象海豹（Mirounga angustirostris）鲸脂转录组组装与注释。本数据集的鲸脂活检样本采集于单次注射30单位促肾上腺皮质激素（adrenocorticotropic hormone，ACTH；2014年10-11月）诱导的急性应激实验期间。分别在ACTH给药前（“基线组”）、给药后2小时（“急性应答组”）及24小时（“恢复组”）采集鲸脂样本。所有样本的平均RNA完整性数值为8.04。文库构建及Illumina HiSeq 2500测序均在加州大学戴维斯分校DNA技术核心实验室按照标准实验流程完成。4份基线组、4份急性应答组及2份恢复组样本在单个Illumina测序泳道上开展100bp双端测序，单样本平均产出3200万条读段，SRA登录号为SRP045540。通过一轮diginorm（khmer v0.8.4；覆盖度：20X，k-mer长度：20）对读段进行质量修剪与丰度标准化处理。使用Trinity v2.0.6默认参数从头组装得到原始转录组。该组装的整体TransRate（v1.0.1）质量得分为0.42，涵盖80%的高度保守脊椎动物同源基因（BUSCOs）。使用TransDecoder v2.0.1构建过滤后的转录组组装，并通过Trinotate v3.0.0生成注释报告（SwissProt数据库下载于2016年3月15日）。