Transcriptome sequencing under cold stress

Low temperature is among the most restrictive factors to limit the yield and distribution of pear. In this study, the mRNA sequencing of P. hopeiensis flower organs exposed to different low temperatures were performed to identify changes of genes and metabolites in response to low-temperature stress. A total of 4 851 differentially expressed genes were identified. Trend analysis showed that these DEGs were significantly enriched in profiles 19, 18, 7, 14, 1, 4 and 11. And the KEGG enrichment analysis showed that the DEGs in profile 18 were significantly enriched in flavone and flavonol biosynthesis. Besides, the expressed trends as well as GO and KEGG functional enrichment analyses of DEGs under cold and freezing stress showed significantly difference.

低温是限制梨属植物产量与分布的最关键制约因素之一。本研究对经不同低温处理的河北梨（P. hopeiensis）花器官开展mRNA测序，以鉴定响应低温胁迫的基因及代谢物变化。本研究共鉴定得到4851个差异表达基因（differentially expressed genes, DEGs）。趋势分析结果显示，上述差异表达基因显著富集于表达谱19、18、7、14、1、4及11中。KEGG（Kyoto Encyclopedia of Genes and Genomes）富集分析表明，表达谱18内的差异表达基因显著富集于黄酮与黄酮醇生物合成通路。此外，冷胁迫与冻胁迫处理下，差异表达基因的表达趋势以及GO（Gene Ontology）、KEGG功能富集分析结果均存在显著差异。