Vibrio vulnificus: Wild type vs. iscR mutant (V. vulnificus iron-sulfur cluster regulator). Vibrio vulnificus MO6-24/O

IscR is a novel global regulator potentially contributing to the overall success in survival and pathogenesis of V. vulnificus by coordinating the regulation of various virulence factors. The profiles of transcripts from the V. vulnificus iscR mutant and the parental wild type were compared by using a V. vulnificus whole-genome microarray. Overall design: Two-condition experiment: Wild type vs. iscR mutant. Biological replicates: 3 control, 3 mutant strains, independently grown and harvested. One replicate per array. For transcriptome analysis, the V. vulnificus whole genome TwinChip, manufactured and kindly provided by the 21C Frontier Microbial Genomics and Applications Center (Daejeon, South Korea), was used.

IscR是一种新型全局调控因子，可通过协调多种毒力因子的表达调控，潜在助力创伤弧菌（Vibrio vulnificus，简称V. vulnificus）的存活与致病过程，提升其整体生存与致病成功率。本研究采用创伤弧菌全基因组微阵列芯片，对比了该菌iscR缺失突变株与其亲本野生型菌株的转录本表达谱。
整体实验设计：双组对照实验，即野生型菌株与iscR突变株的对比。
生物学重复：对照组与突变株组各设置3株独立培养并收获的菌株，每块芯片对应一个生物学重复。
本转录组分析所用的创伤弧菌全基因组TwinChip芯片，由韩国大田市21C前沿微生物基因组与应用中心研发并惠赠。