Variant-specific vaccination induces systems immune responses and potent in vivo protection against SARS-CoV-2 [scVDJ-seq]

We generated LNP-mRNAs specifically encoding wildtype (WT), B.1.351 and B.1.617 SARS-CoV-2 spikes, and systematically studied their immune responses. Single cell BCR-seq and TCR-seq unveiled repertoire diversity and clonal expansions in vaccinated animals. Overall design: Splenocytes were isolated and processed from immunized mice and analyzed by scVDJ-seq.

本研究构建了专门编码野生型（WT）、B.1.351株与B.1.617株严重急性呼吸综合征冠状病毒2（SARS-CoV-2）刺突蛋白的脂质纳米颗粒信使核糖核酸（LNP-mRNA），并系统研究了其诱导的免疫应答。单细胞B细胞受体测序（Single cell BCR-seq）与T细胞受体测序（TCR-seq）揭示了免疫动物体内的免疫组库多样性与克隆扩增特征。实验整体设计：从免疫小鼠体内分离脾脏淋巴细胞并完成处理，通过单细胞VDJ测序（scVDJ-seq）开展分析。