Summary of all analyzed probes in this study.

(A) and (B) Comparison of HSE results for all probe sets with distance to the STR marker, G/C content, hit frequency, and free energy differences of match and mismatch in kcal/mol.1The number is the distance in kb of strong hits to the extraction locus, + indicates the presence of further hits but greater distance (>30 kb).2Best simulated probes P38FA (11 nucleotides long) and P38FA-1 (12 nucleotides long) show many hits (4, 12, 16 kb+ and 4, 10 16 kb+).3HSE results show the best experimentally evaluated probe length with enrichment of one contributor. Low separation in HSE could be due to a higher hit frequency close to the extraction locus and great distance from the STR marker. Asterix indicate the best probe length. (C) The best probe length was evaluated after simulation and chosen after highest Δcon, and comparison of the prediction for best probe length after Δcon and best probe in HSE: (r) right prediction, (w) wrong prediction. (D) Listing of all analyzed match and mismatch constellations in this study. List of complete data is available in Data S3.

(A)与(B) 对比了所有探针组的HSE（Hybridization Selection Efficiency）结果，涵盖与STR（Short Tandem Repeat）标记的距离、G/C碱基占比、命中频率，以及匹配与错配的自由能差（单位：千卡每摩尔，kcal/mol）。1 数值表示强命中位点至提取位点的距离（单位：千碱基对，kb）；符号"+"代表存在其他命中位点，但二者距离更远（>30 kb）。2 最优模拟探针P38FA（长度为11个核苷酸）与P38FA-1（长度为12个核苷酸）均存在多个命中位点，分别为4、12、16 kb+与4、10、16 kb+。3 HSE结果显示，经实验评估的最优探针长度存在单一贡献因子富集。HSE分离度较低的原因可能为：靠近提取位点的命中频率更高，且与STR标记的距离更远。星号用于标注最优探针长度。(C) 最优探针长度经模拟评估后，以最高Δcon值为标准进行筛选；同时对比了基于Δcon筛选出的最优探针长度与HSE筛选出的最优探针：其中(r)代表预测正确，(w)代表预测错误。(D) 本研究中所有分析的匹配与错配组合列表。完整数据集可参见Data S3。