Gene network analysis for identification of microRNA biomarkers for asthma [Lung_RNASeq]. Gene network analysis for identification of microRNA biomarkers for asthma [Lung_RNASeq]

To identify relevant mRNA targets of differentially dysregulated lung miRNA signatures of a house dust mite mouse (HDM) model of asthma. cDNA libraries were constructed using the same lung RNA samples as the miRNA libraries preparation. Overall design: Lung mRNA profile from HDM-exposed mice (n=16) was compared to the profile of Saline-treated (controls, n=14) mice.

本研究旨在鉴定哮喘屋尘螨（house dust mite, HDM）小鼠模型中肺部差异失调的微小RNA（microRNA, miRNA）特征的相关信使核糖核酸（messenger RNA, mRNA）靶标。本研究使用与制备微小RNA文库相同的肺部核糖核酸（ribonucleic acid, RNA）样本构建了互补脱氧核糖核酸（complementary DNA, cDNA）文库。实验设计：将暴露于屋尘螨的小鼠（n=16）的肺部mRNA表达谱与经生理盐水处理的对照组小鼠（n=14）的表达谱进行对比。