Transcriptome sequencing of myelodysplasia

Transcriptome sequencing was performed on 214 patients with myelodysplasia in this study. RNA was obtained from bone marrow CD34+ cells (n=100) and/or bone marrow mononuclear cells (n=165). Transcriptome sequencing was performed for both cell fractions in 51 patients. We also studied bone marrow CD34+ cells and bone marrow mononuclear cells obtained from three healthy adults each.

本研究对214例骨髓增生异常综合征（myelodysplasia）患者进行了转录组测序（transcriptome sequencing）。实验所用RNA分别取自骨髓CD34阳性细胞（bone marrow CD34+ cells，n=100）及/或骨髓单个核细胞（bone marrow mononuclear cells，n=165）；其中51例患者同时对这两类细胞组分实施了转录组测序。此外，本研究还分别采集3名健康成人的骨髓CD34阳性细胞与骨髓单个核细胞开展相关研究。