High-protein intake eliminates the weight loss-induced improvement in insulin sensitivity. Homo sapiens

The purpose of this study was to conduct a randomized-controlled trial to evaluate the effect of a high-protein (HP) diet weight loss (WL) on changes in body composition, insulin sensitivity and skeletal muscle gene expression profile in postmenopausal women who were obese and randomized to one of three dietary intervention groups: 1) a weight maitenance group, 2) a weight loss group (normal protein intake, 0.8 g protein/kg body weight per day) and 3) a weight loss group (high protein intake, 1.2 g protein/kg body weight per day) and studies before and after they lost 10% of their body weight (WL groups) or a time-matched weight maintenacne period. Overall design: We examined skeletal muscle samples obtained from two groups; 1) 9 subjects in a WL-HP group and 2) 6 subjects in a WL-NP group before and after 10% weight loss (i.e. the weight maitenance group was not included in the current microarray study).

本研究旨在开展一项随机对照试验，评估高蛋白（high-protein, HP）饮食减重（weight loss, WL）方案对肥胖绝经后女性身体成分、胰岛素敏感性及骨骼肌基因表达谱的影响。所有受试者被随机分配至3组膳食干预方案：1）体重维持组，2）正常蛋白摄入减重组（每日蛋白质摄入量为0.8g/kg体重），3）高蛋白摄入减重组（每日蛋白质摄入量为1.2g/kg体重）。研究分别对减重组受试者体重下降10%前后，以及体重维持组受试者时间匹配的体重维持阶段前后开展检测。整体实验设计：本研究检测了两组受试者的骨骼肌样本：1）高蛋白摄入减重组（WL-HP）共9名受试者，2）正常蛋白摄入减重组（WL-NP）共6名受试者，分别采集其体重下降10%前后的样本；本项微阵列研究未纳入体重维持组受试者。