Chromatin remodeling in HIF-1a deficient B cell during IgA class switching [ChIP-seq]

The interconnection between metabolic programming and chromatin remodeling during IgA class switching suggest that HIF-1a-dependent glycolytic flux may control the epigenetic modification at a class switching region (Sa) in the Igh locus. To test our hypothesis, histone acetylation was analyzed by chromatin immunoprecipitation sequencing (ChIP-seq) (H3K9ac and H3K27ac) in Hif-1a deficient and control B cell during IgA class switching. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K27ac and H3K9ac in B cell from WT and HIF-1a deficient mice. Overall design: 6 WT samples and 6 HIF-1a deficient samples for ChIP-seq.

IgA类别转换过程中，代谢重编程与染色质重塑之间的相互关联提示，缺氧诱导因子1α (HIF-1α) 依赖的糖酵解通量可能调控免疫球蛋白重链基因座（Igh locus）内类别转换区域（Sa）的表观遗传修饰。为验证本研究假说，我们在IgA类别转换阶段的HIF-1α缺陷型与对照B细胞中，通过染色质免疫共沉淀测序（ChIP-seq）分析了组蛋白H3K9ac与H3K27ac的乙酰化修饰水平。本数据集包含来自野生型与HIF-1α缺陷型小鼠B细胞的、针对组蛋白修饰H3K27ac与H3K9ac的染色质免疫共沉淀测序数据。实验整体设计：共计6份野生型样本与6份HIF-1α缺陷型样本用于ChIP-seq检测。