Optimisation and benchmarking of targeted amplicon sequencing for mycobiome analysis of respiratory specimens

We performed ITS analysis of respiratory (sputum) samples by assessing (a) the effect of alternate DNA extraction techniques and (b) an evaluation of four different ITS primer pairs (ITS1F and ITS2; ITS1-30F and ITS1-217R; gITS7ngs and ITS4ng; Fseq and Rseq) on the mycobiome profiles generated for mock fungal communities and their respective clinical (airway) specimens.

本研究针对呼吸道（痰液）样本开展内部转录间隔区（Internal Transcribed Spacer, ITS）分析，通过两项评估考察相关因素对真菌组（Mycobiome）谱型的影响：(a) 替代性DNA提取技术的应用效果；(b) 四款不同的ITS引物对[ITS1F与ITS2、ITS1-30F与ITS1-217R、gITS7ngs与ITS4ng、Fseq与Rseq]，对为模拟真菌群落及其对应临床（气道）标本所生成的真菌组谱型所产生的影响。