CD146+CD45-CD31- pericyte RNA sequencing following an acute bout of electrical stimulation

The objective of this study was to understand the mechanisms by which electrical stimulation in vivo leads to improvements and growth in skeletal muscle post exercise through changing the function of CD146+CD45-CD31- pericytes. We utilized global gene profiling to identify novel signaling pathways and patterns of gene expression involved in this adaptation process. RNA was extracted from CD146+CD45-CD31- pericytes that were isolated from the gastrocnemius muscles of male mice 3 hours post an acute 10 minute bout of electrical stimulation. Mice that had been electrically stimulated were compared to sham control mice. Mice were equally distributed among the two groups with n=3/group

本研究旨在阐明体内电刺激通过调控CD146+CD45-CD31-周细胞（pericytes）的功能，进而改善运动后骨骼肌状态并促进其生长的具体机制。我们采用全基因表达谱分析技术，鉴定参与该适应性过程的新型信号通路与基因表达模式。我们从经10分钟急性电刺激3小时的雄性小鼠腓肠肌中分离得到CD146+CD45-CD31-周细胞，并提取该类细胞的总RNA。将接受电刺激的实验组与假手术对照组小鼠进行对照分析，两组均按每组3只（n=3/组）的规则进行均衡分配。