RNA-seq of HTR-8/SVneo cells with MELTF-AS1 knockdown

The study includes two experimental conditions: 1. **MELTF-AS1 knockdown group:** HTR-8/SVneo cells transfected with specific siRNAs targeting the MELTF-AS1 gene.2. **Control group:** HTR-8/SVneo cells treated with non-targeting scramble siRNA. The goal of this project is to identify differentially expressed genes (DEGs) and dysregulated signaling pathways resulting from MELTF-AS1 loss-of-function. As HTR-8/SVneo cells are a well-established model for studying trophoblast invasion and placentation, this data provides a valuable resource for understanding the potential role of MELTF-AS1 in the molecular etiology of pregnancy-related disorders, such as pre-eclampsia, where inadequate trophoblast invasion is a key pathological feature. Researchers can use this dataset to explore lncRNA-mediated regulatory networks in placental development and disease.

本研究设置两组实验条件：1. **MELTF-AS1敲低组**：采用靶向MELTF-AS1基因的特异性小干扰RNA（small interfering RNA, siRNA）转染HTR-8/SVneo细胞。2. **对照组**：采用非靶向乱序siRNA处理HTR-8/SVneo细胞。本项目旨在鉴定MELTF-AS1功能缺失所诱导的差异表达基因（differentially expressed genes, DEGs）及失调信号通路。鉴于HTR-8/SVneo细胞是研究滋养细胞侵袭与胎盘形成的经典模型，本数据集可为解析MELTF-AS1在子痫前期（pre-eclampsia）等妊娠相关疾病的分子病因学中的潜在作用提供宝贵资源——子痫前期以滋养细胞侵袭不足为核心病理特征。研究人员可依托本数据集探索长链非编码RNA（long non-coding RNA, lncRNA）介导的胎盘发育与疾病相关调控网络。