Genome-wide profiling of DNA hydroxymethylation patterns in iAs-transformed cells. Homo sapiens

To examine the global impact of iAs on DNA hdroxymethylation patterns. Genomic DNA was glucosylated, digested and analyzed using Reduced Representation Hydroxymethylation Profiling. Overall design: DNA from both NT and iAs-transformed cells were glucosylated, digested and analyzed using Reduced Representation Hydroxymethylation Profiling.

为探究无机砷（iAs）对DNA羟甲基化（DNA hydroxymethylation）图谱的全局影响，研究人员对基因组DNA进行糖基化、酶切处理，并采用简化代表性羟甲基化谱分析（Reduced Representation Hydroxymethylation Profiling）完成检测。
实验设计：分别提取NT细胞与iAs转化细胞的基因组DNA，经糖基化、酶切处理后，采用简化代表性羟甲基化谱分析进行检测分析。