SH1410938.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE平台为界定、识别、交流及处理基于DNA的物种假说（Species Hypotheses，以下简称SH）提供了统一的标准化方法。国际核酸序列数据库中的所有真菌内转录间隔区（Internal Transcribed Spacer，ITS）序列，通过应用一套动态距离阈值（0.5%~3.0%）被聚类至近似物种级别的单元。所有物种假说均以数字对象标识符（Digital Object Identifier，DOI）的形式赋予唯一且稳定的名称，其分类学与生态学注释信息则通过分布式的网络第三方注释工作完成验证。每个物种假说都会结合该单元内全部序列的鉴定结果，尽可能关联至对应的分类群名称及其分类层级（如门、纲、目等）。科研人员会为每个物种假说选取一条经自动或人工标定的序列作为其代表序列。这些代表序列已通过（https://unite.ut.ee/repository.php）对外发布，供科研群体用于本地序列相似性搜索、下一代测序分析流程等科研场景。随着公共真菌ITS序列数量的不断增长，该系统与配套数据会自动进行更新。