RCOR2 downregulates CIITA via HDAC1-mediated histone deacetylation in cancer cells [ChIP-seq]

RCOR2 and HDAC1 directly co-binds to CIITA gene. RCOR2 knockout does not affect HDAC1 binding on CIITA gene. RCOR2 has no binding on most MHCII genes. MDA-MB-231 cells overexpressing HA tagged RCOR2 were subjected to ChIP-seq with anti-HA antibody. Parental and RCOR2-KO MDA-MB-231 cells were subjected to ChIP-seq with anti-HDAC1 antibody. Occupancy of RCOR2 and HDAC1 on CIITA and MHCII genes were analyzed.

REST共抑制因子2（RCOR2）与组蛋白去乙酰化酶1（HDAC1）可直接共同结合于Ⅱ类主要组织相容性复合体反式激活因子（CIITA）基因。RCOR2基因敲除不会影响HDAC1在CIITA基因上的结合。RCOR2几乎不结合绝大多数主要组织相容性复合体Ⅱ类（MHCII）基因。对过表达血凝素标签（HA tag）RCOR2的MDA-MB-231细胞，采用抗HA抗体进行染色质免疫共沉淀测序（ChIP-seq）；对亲本野生型及RCOR2基因敲除的MDA-MB-231细胞，采用抗HDAC1抗体进行ChIP-seq分析，并对RCOR2与HDAC1在CIITA基因及MHCII基因上的结合占有率进行了分析。