Iterative Relay of EGFR-ERK Signaling Regulates Epithelial Invagination via a Wave of Cellular Contractility

Activity of ERK FRET biosensor was was quantified from time-lapse images of Drosophila embryos carrying pubi-ekarev-nes and pubi-TagRFP-T-CAAX. TagRFP-T-CAAX signal was used to segment cells and averaged FRET/CFP ratio and position (in polar coordinate) or time of each segmented cell is listed in each table. Those data set were used to create the graphs in Figure 1 of Ogura et. al.(2018).

本数据集针对携带pubi-ekarev-nes与pubi-TagRFP-T-CAAX的果蝇胚胎的延时显微图像，对ERK荧光共振能量转移生物传感器（ERK FRET biosensor）的活性进行了定量分析。以TagRFP-T-CAAX信号完成细胞分割，各表格均记录了每个分割细胞的平均FRET/CFP比值、极坐标系下的位置信息，或对应的时间节点。该数据集被用于生成Ogura等人（Ogura et al., 2018）论文图1中的相关图表。