Recombinant irisin enhances the differentiation, remodeling potential, and the mechanical properties of 3D-cultured human periodontal ligament cells

Irisin is recognized as a myokine produced by muscles, regulating metabolism and energy homeostasis, however, it may play a role in many other biological functions. Little is known about its effect on periodontal ligament cells. We employed Affymetrix to profile mRNA expression patterns between 3D human periodontal ligament cell spheroids treated with and without irisin. The mRNA expression profiling identified approximately 1000 mRNAs to be differentially expressed between the two groups, which suggests that irisin is involved in gene regulation in human periodontal ligament cells. The 3D cultured human periodontal ligament cells were treated with 10 ng/ml irisin or miliQ water (control) for 14 days, total RNA was extracted from 3 replications from each group and sent for Affymetrix.

鸢尾素（Irisin）是一种由肌肉分泌的肌因子（myokine），可调控机体代谢与能量稳态，同时还可能参与诸多其他生物学功能。目前对于其在牙周膜细胞（periodontal ligament cells）中的作用尚缺乏深入研究。本研究采用Affymetrix芯片技术，对经鸢尾素处理与未处理的三维人牙周膜细胞球状体的mRNA表达模式进行了分析。通过mRNA表达谱鉴定，两组样本间共存在约1000个差异表达mRNA，提示鸢尾素可参与人牙周膜细胞的基因调控过程。本实验中，三维培养的人牙周膜细胞分别以10 ng/ml鸢尾素或Milli-Q超纯水（对照组）处理14天，随后从每组的3个生物学重复样本中提取总RNA，送至Affymetrix平台开展表达谱检测。