Next Generation Sequencing of Saline and Methamphetamine-treated Mouse Hippocampus Transcriptomes

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived hippocampus transcriptome profiling (RNA-seq) in mouse to evaluate alterations in hippocampus with methamphetamine administrations. Methods: Hippocampus mRNA profiles of Control and Methamphetamine-treated mice were generated by deep sequencing, in triplicate, using Illumina Sequencing Platform.

研究目标：下一代测序（Next-generation sequencing, NGS）已革命性地推动了系统层面的细胞通路分析。本研究旨在对比基于NGS的小鼠海马体转录组谱（RNA-seq），以评估甲基苯丙胺给药后海马体的转录组变化。实验方法：本研究采用Illumina测序平台，通过深度测序并设置三次生物学重复，获得了对照组与甲基苯丙胺处理组小鼠的海马体mRNA表达谱。