Human betaherpesvirus 6A strain:U1102 Transcriptome or Gene expression. Human betaherpesvirus 6A strain:U1102

We report the application of size sele selection of small RNA species isolated from Jjhan cells harboring the human herpesvirus 6A genome. We ammassed >3.4million reads of sequence from three different sources: Normal Brain cell total RNA, Jjhan total RNA and HHV-6A BAC transfected Jjhan total RNA. Sequences were mapped to the HHV-6A Uganda 1102 strain genome (GenBank: X83413.1) with no less than 100% match for reads >20nt and <23nt. The resulting pool of candidates was mapped to the HHV-6A genome.

本研究报道了从携带人类疱疹病毒6A型（human herpesvirus 6A, HHV-6A）基因组的Jjhan细胞中分离得到的小RNA分子的大小筛选应用。本研究从三类不同样本中累计获得超过340万条序列读段，样本分别为正常脑细胞总RNA、Jjhan细胞总RNA，以及经HHV-6A细菌人工染色体（Bacterial Artificial Chromosome, BAC）转染的Jjhan细胞总RNA。将长度大于20nt（nucleotide, 核苷酸）且小于23nt的序列读段比对至HHV-6A Uganda 1102毒株基因组（GenBank: X83413.1），比对匹配度不低于100%。最终得到的候选序列集被进一步比对至HHV-6A基因组。