Hepatic Glucocorticoid Receptor (GR) drives the diurnal adaptation to Caloric Restriction

We examined the gene expression profiles of liver samples throughout the day/night cycle (ZT0-ZT4-ZT8-ZT12-ZT16-ZT20) from control mice (night-restricted fed) and mice under short-term caloric restriction. Overall design: Bulk RNA sequencing (RNA-seq) from livers of WT and hepatocyte-deleted for Glucocorticoid Receptor mice (GR liver KO ) upon night restricted feeding (NR) and caloric restriction (CR) with sampling every 4hours at 6 different timepoints (ZT0, ZT4, ZT8, ZT12, ZT16, and ZT20), ZT corresponds to Zeitgeber Time, 6 a.m :ZT0 & ZT12: 6 p.m. Additionally, bulk RNA sequencing (RNA-seq) from livers of night-restricted fed (NR) and caloric restricted (CR) WT mice at Zeitgeber 12 (ZT12; 6 p.m).

本研究分析了昼夜周期（ZT0-ZT4-ZT8-ZT12-ZT16-ZT20）内，夜间限食对照组小鼠与短期热量限制小鼠的肝脏基因表达谱。总体实验设计：针对野生型（wild type，WT）和肝细胞糖皮质激素受体（Glucocorticoid Receptor，GR）敲除（GR liver KO）小鼠，分别在夜间限食（night-restricted fed，NR）与热量限制（caloric restriction，CR）条件下，于6个时间点（每4小时采样一次，即ZT0、ZT4、ZT8、ZT12、ZT16、ZT20）采集肝脏组织，开展批量RNA测序（bulk RNA sequencing，RNA-seq）；其中ZT即授时因子时间（Zeitgeber Time），ZT0对应上午6时，ZT12对应下午6时。此外，本研究还对夜间限食（NR）与热量限制（CR）的野生型（WT）小鼠，在授时因子12（ZT12，即下午6时）时采集肝脏组织进行批量RNA测序。