Additional file 2 of Genome-wide characterization and expression analysis of the heat shock transcription factor family in pumpkin (Cucurbita moschata)

Additional file 2: Figure. S1. Multiple sequence alignment analysis and the secondary structure elements of DBD in CmHsf proteins. Sequence alignments were performed using Clustal X 2.0. Different background colors indicated different amino acids. “*” meant that the amino acid sequences of different Hsf proteins are highly consistent. The secondary structure elements of DBD (α1-β1-β2-α2-α3-β3-β4) were shown above the alignment. The secondary structure was predicted by SOPMA secondary structure prediction software. Cylindrical tubes represented a-helices or β-sheets. Figure. S2. Detailed information about the 10 motifs identified in CmHsf proteins. The LOGOs of the protein motifs were also obtained with Multiple Expectation Maximization or Motif Elicitation (MEME, http://meme-suite.org/ ).

附加文件2：图S1。CmHsf蛋白中DNA结合域（DNA-binding domain, DBD）的多序列比对分析及其二级结构元件。序列比对通过Clustal X 2.0软件完成。不同背景色对应不同的氨基酸残基；符号“*”表示不同Hsf蛋白的氨基酸序列高度保守。DBD的二级结构元件（α1-β1-β2-α2-α3-β3-β4）标注于比对序列上方，该二级结构由SOPMA二级结构预测软件完成预测，其中圆柱形线条代表α螺旋或β折叠片层。

图S2。CmHsf蛋白中鉴定得到的10个基序的详细信息。蛋白质基序的序列Logo图通过多重期望最大化或基序发现工具（Multiple Expectation Maximization or Motif Elicitation, MEME, http://meme-suite.org/）获取。