Myeloid Derived Suppressor Cells and Cancer. Mus musculus

Transcriptional profiling of FACS-sorted and splenic control mouse cells, comparing splenic cells from FVBneuN vs Neu+ expressing FVBneuN mice with Gr1+ CD11b+ sorted tumor-infiltrating mononuclear or splenic myeloid-derived suppressor cells Overall design: 4 groups or conditions. Biological replicates: 2 or 3 per condition. One replicate array per sample. manuscript: van Deventer, H, J Burgents, QP Wu, R Woodford, WJ Brickey, I Allen, E McElvania-Tekippe, J Serody, and J Ting. (2010) The inflammasome component Nlrp3 impairs antitumor vaccine by enhancing the accumulation of tumor-associated myeloid-derived suppressor cells. Cancer Research. variable: cell type: splenic cells from normal FVB-neuN mice, splenic cells from Neu+ tumor-bearing FVB-neuN mice, Gr1+ CD11b+ sorted cells from tumor, Gr1+ CD11b+ sorted cells from spleen repear: biological replicate: #1, #2, #3

本数据集针对经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）分选的细胞及脾脏对照小鼠细胞开展转录组谱分析，对比分析FVBneuN小鼠脾脏细胞、表达Neu+的FVBneuN小鼠脾脏细胞，以及经Gr1+ CD11b+分选的肿瘤浸润单核细胞或脾脏髓系来源抑制细胞（myeloid-derived suppressor cells, MDSC）。实验设计：共设置4组实验条件，每组生物学重复数为2或3，每个样本对应一张基因芯片（array）。相关研究参考文献：van Deventer H、Burgents J、Wu QP、Woodford R、Brickey WJ、Allen I、McElvania-Tekippe E、Serody J与Ting J于2010年发表于《Cancer Research》的论文，原英文标题为"The inflammasome component Nlrp3 impairs antitumor vaccine by enhancing the accumulation of tumor-associated myeloid-derived suppressor cells"，中文译名为《炎症小体（inflammasome）组分Nlrp3通过促进肿瘤相关髓系来源抑制细胞积累削弱抗肿瘤疫苗功效》。实验变量为细胞类型，具体包含以下四类：正常FVB-neuN小鼠脾脏细胞、携带Neu+肿瘤的FVB-neuN小鼠脾脏细胞、肿瘤来源的Gr1+ CD11b+分选细胞、脾脏来源的Gr1+ CD11b+分选细胞。实验重复信息：采用生物学重复，重复编号为#1、#2、#3。