Fertilization can accelerate the pace of soil microbial community response to rest-grazing duration in the Three-river Source Region of China

Objectives
Overgrazing leads to grassland degradation and productivity decline. Rest-grazing during the regreen-up period can quickly restore grassland and fertilization is a common restoration measure. Meanwhile, soil microorganisms are more sensitive indicators. Therefore, the experiment of rest-grazing time and fertilization was carried out to explore the response of soil microorganisms to rest-grazing time and fertilization measures.
Methods
A field control experiment with rest-grazing time and fertilization as factors was conducted from the time when grass returned to green till the livestock moved to the summer pasture in Dawu Town of Maqin County. The primary treatment we established was the five rest-grazing times, including rest-grazing times of 20 days, 30 days, 40 days, 50 days, and traditional grazing was used as a check group. At the same time, the secondary treatment was nitrogen addition of 300 kg·hm-2 in each primary treatment.
Results
The results showed that: the..., Soil samples were obtained in August 2017, when the grass was growing vigorously. In each subplot, three soil samples were collected to reduce spatial heterogeneity, and five soil cores (3.5cm diameter) were taken at a depth of 0 to 15 cm regarding each one as one soil sample.  After the soil samples were measured, the data of three soil samples from each subplot were averaged to obtain one value. Therefore, only one value was obtained for each subplot. In total, three independent samples were obtained from each treatment of three replicate blocks (A, B and C). In order to avoid the influence of livestock waste on soil characteristics, we absolutely avoid livestock waste and the traces of livestock waste when sampling. A total of 90 soil samples (three soil samples ×ten subplots × three replicate blocks) were collected in this study. Then, three cutting ring soil samples were collected from 0–15 cm depths in each plot to determine the soil moisture content (SM). In addition, when collec..., , This README file was generated on 2023-11-14 by Xuanbo Zhou.

GENERAL INFORMATION

1. Title of Dataset: Fertilization can Accelerate the Pace of Soil Microbial Community Response to Rest-grazing Duration in the Three-river Source Region of China
2. Author Information
A. Principal Investigator Contact Information
Name: Xuanbo Zhou
Institution: Qinghai University
Address: Xining,Qinghai, OR China
Email: [e-mail]

B. Corresponding author Contact Information
Name:Xiaoli Wang
Institution: Qinghai University
Address: Xining,Qinghai, OR China
Email: [e-mail]
3. Date of data collection (single date, range, approximate date): July 2017 - August 2017
4. Geographic location of data collection: Yongbao Animal Husbandry Committee, Dawu Town, Guoluo Tibetan Autonomous Prefecture
5. Information about funding sources that supported the collection of the data: National Natural Science Foundation of China (32260327 ,32230068 & U21A20183) and t...

目标
过度放牧会导致草地退化和生产力下降。返青期休牧（rest-grazing）可快速恢复草地，而施肥（fertilization）是常用的恢复措施。同时，土壤微生物是更为敏感的指示指标。因此，本研究开展了休牧时间与施肥的试验，旨在探究土壤微生物对休牧时间及施肥措施的响应。
方法
以休牧时间和施肥为影响因子，于玛沁县大武镇草地返青至牲畜转入夏季牧场期间开展野外控制试验。试验设置的主处理为5个休牧时长，包括20天、30天、40天、50天休牧，同时以传统放牧作为对照。次处理为在各主处理中添加300 kg·hm⁻²的氮肥。
结果
研究结果显示：... 土壤样品于2017年8月草地生长旺盛期采集。为降低空间异质性，每个亚样地采集3份土壤样品，每份样品由5个直径3.5 cm、深度0-15 cm的土芯混合而成。土壤样品测定后，每个亚样地的3份样品数据取平均值作为该亚样地的代表值，因此每个亚样地仅获得1个数据值。每个处理在3个重复区块（A、B、C）中各得到3个独立样品。采样时为避免牲畜粪便对土壤特性的影响，严格避开粪便及其痕迹。本研究共采集90份土壤样品（3份样品×10个亚样地×3个重复区块）。此外，每个样地采集3份0-15 cm深度的环刀土样，用于测定土壤含水量（SM）。另外，在采集... 本README文件由周轩波于2023年11月14日生成。
基本信息
1. 数据集标题：施肥可加速三江源区土壤微生物群落对休牧时长的响应速率
2. 作者信息
A. 首席研究员联系方式
姓名：周轩波
单位：青海大学
地址：中国青海省西宁市
邮箱：[e-mail]

B. 通讯作者联系方式
姓名：王小丽
单位：青海大学
地址：中国青海省西宁市
邮箱：[e-mail]
3. 数据采集日期：2017年7月-2017年8月
4. 数据采集地理位置：果洛藏族自治州大武镇永保畜牧委员会
5. 支持数据采集的 funding 来源信息：国家自然科学基金（32260327、32230068及U21A20183）及...