Exome-Seq of murine MB49 cell line - WT and gal-9-KO - in vitro

A number of studies have reported evidence of positive or negative contributions of galectin-9 (gal-9) to human and experimental malignancies. Some clinical observations and in vitro experiments suggest that cell-associated gal-9 has anti-metastatic effects. On the other hand, extra-cellular gal-9 consistently enhances tumor immune escape. The aim of this experiment is to study the impact of gal-9 gene invalidation in the murine bladder carcinoma cell line MB49, in vitro. It complements the results of : 1) a RNAseq analysis performed on in vitro cell cultures ; 2) a comparative experiment carried out using the same cells as part of a serial transplantations protocol, whose results are already available on Annotare (E-MTAB-9570 ; E-MTAB-9220).

已有多项研究报道半乳糖凝集素9（galectin-9，gal-9）对人类及实验性恶性肿瘤存在正向或负向调控效应。部分临床观察与体外实验结果显示，细胞结合型gal-9具有抗转移活性；与之相对，细胞外gal-9则可持续促进肿瘤免疫逃逸。本实验旨在体外探究gal-9基因失活对小鼠膀胱癌细胞系MB49的影响，并补充以下两项研究的结果：1）针对体外细胞培养样本开展的RNA测序（RNAseq）分析；2）采用该细胞系开展连续移植实验方案中的对照实验，其相关结果已在Annotare数据库公开（编号：E-MTAB-9570、E-MTAB-9220）。