3DED/microED datasets of biotin (Glacios/Ceta-D)

3DED/microED data collected from 11 crystals of biotin (Sigma-Aldrich 14400) using a Thermo Fisher Scientific Glacios microscope equipped with a Ceta-D Camera. An image of the grid square with the crystal used for data collection marked is included in each folder.

Data collection settings:

200kV, microprobe, gun lens 4, spot size 11, 30 µm C2 aperture. Illuminated area was ~3.3 µm and a 40 µm SA aperture was used. Camera length calibrated from Al powder is 838.5 mm. Data acquired using EPU-D (v1.5) with following settings: 2x binning, 0.5 °/s, 2s exposure, rolling shutter, noise reduction mode enabled. 

MRC format images can be processed with DIALS using the FormatMRC dxtbx format (distributed with DIALS from version 3.5 onwards), use goniometer.axes=1,0,0.

本数据集包含从11颗生物素（biotin，Sigma-Aldrich货号14400）晶体采集的三维电子衍射（3DED）与微区电子衍射（microED）数据，采集设备为搭载Ceta-D探测器（Ceta-D Camera）的赛默飞世尔科技（Thermo Fisher Scientific）Glacios透射电子显微镜。每个文件夹均附带一张标注了数据采集所用晶体的网格方格图像。

数据采集参数如下：

加速电压200kV，采用微探针模式，枪透镜参数设为4，光斑尺寸11，配置30 µm的C2光阑。照明区域面积约3.3 µm，使用40 µm的选区光阑（SA）。通过铝粉标定得到的相机长度为838.5 mm。数据采集通过EPU-D（v1.5）软件完成，具体设置为：2x像素合并，转速0.5 °/s，曝光时长2秒，启用卷帘快门模式与降噪功能。

MRC格式图像可通过DIALS软件调用FormatMRC dxtbx格式接口进行处理（自DIALS 3.5版本起该格式随软件同步分发），需设置参数goniometer.axes=1,0,0。