Appraisal of acute oral toxicity of glucuronoxylan hydrogel from Mimosa pudica seeds

Glucuronoxylan hydrogel (GXH) isolated from M. pudica seeds was assessed for acute toxicology in albino mice that were alienated into four groups. Three groups, i.e., II, III and IV received GXH at a dose of 1, 2 and 5 g/kg, respectively while group I was retained untreated and provided routine diet. After administering GXH, mice were examined for vomiting, diarrhea, allergy and tremors for 8 h. All animals were carefully observed for food and water consumption at 1, 2, 3, 7 and 14 day after administering GXH. At the end of studies, blood samples were drawn for investigation of hematological and biochemical parameters. All animals were sacrificed, relative body weight of vital organs was calculated and their histopathology was studied. It was concluded that there was insignificant difference in body weight, behavioral pattern, food and water intake among treated and control groups. Haematology and biochemistry of blood samples from all groups were found analogous. Histopathological evaluation of vital body organs exhibited no lesions in all groups. Ocular, cardiac and dermal safety of GXH was also established on albino rabbits.

从含羞草（M. pudica）种子中提取的葡萄糖醛酸木聚糖水凝胶（Glucuronoxylan hydrogel, GXH），以白化小鼠为模型开展了急性毒性试验。实验将小鼠分为四组，其中第II、III、IV组分别以1、2、5 g/kg的剂量给予GXH，第I组为空白对照组，不予处理并给予常规饲料与饮水。给药后8小时内，对小鼠的呕吐、腹泻、过敏反应及震颤情况进行观察；于给药后1、2、3、7、14天，记录各组小鼠的摄食量与饮水量。实验结束后，采集血液样本用于血液学与生化指标检测；处死所有实验动物，计算各重要脏器的相对体重并开展组织病理学研究。经分析得出结论，给药组与对照组在体重、行为模式、摄食量及饮水量方面均无显著性差异；各组血液样本的血液学及生化指标结果相近；重要脏器的组织病理学评估未发现任何病变。此外，本研究还在白化家兔上验证了GXH的眼部、心脏及皮肤安全性。