Analysis of Colletotrichum orbiculare infection of N. benthamiana

Species from the genus Colletotrichum are the causal agents of anthracnose which contribute to significant losses to the production of commercially grown crops. The genomes of Colletotrichum orbiculare, which infects cucurbits and Nicotiana benthamiana, as well as Colletotrichum gloeosporioides, which infects a wide range of fruits and vegetables, were sequenced. A custom microarray was designed for Colletotrichum orbiculare and used to assess gene expression during infection of Nicotiana benthamiana. Gene expression of Colletotrichum orbiculare growing on its host Nicotiana benthamiana was assessed at 24 hours post inoculation, 3 days post inoculation and 7 days post inoculation. Mycelia growing in vitro and ungerminated conidia were used as controls. Three replicates were performed for each time point. Pamela, Gan

刺盘孢属（Colletotrichum）物种是引发炭疽病的病原菌，其对商业化栽培作物的生产造成了严重损失。本研究对侵染葫芦科植物与本氏烟草（Nicotiana benthamiana）的瓜类刺盘孢（Colletotrichum orbiculare），以及侵染多种果蔬的胶孢刺盘孢（Colletotrichum gloeosporioides）的基因组进行了测序。研究人员针对瓜类刺盘孢设计了定制化基因芯片（microarray），用于分析其侵染本氏烟草过程中的基因表达模式。本研究分别在接种后24小时、3天及7天三个时间点，对寄生于本氏烟草的瓜类刺盘孢的基因表达水平进行了检测；以体外培养的菌丝体与未萌发的分生孢子作为对照样本。每个时间点均设置三次生物学重复实验。作者：Pamela, Gan