Transcriptomic profiling of boars with high and low skatole levels. Sus scrofa

Skatole is one of the compounds causing boar taint in meat of uncastrated male pigs. This study focuses on differences in gene expression of 50 Danish Landrace boars with high and low skatole levels. The animals were divided into 2 groups based on the skatole measurements, with animals having a skatole concentration of less than 0.01 ppm in one group, and above 0.275 ppm in the other group. Gene expression was assessed using porcine oligonucleotide microarrays. We report differential expression of CYP2E1, CYP2A13 and suggests involvement of FMO1, HSD17B13, CBR4 and several transcription factors. Overall design: Gene expression study of liver, comparison of 25 boars with high and 25 boars with low skatole levels, using in-house printed porcine two-colour oligonucleotide microarrays.

粪臭素（skatole）是引发未阉割公猪肉类产生公猪膻味的化合物之一。本研究聚焦于50头丹麦长白公猪中粪臭素水平高低两组间的基因表达差异。研究人员基于粪臭素检测结果将受试动物分为两组：一组粪臭素浓度低于0.01 ppm，另一组浓度高于0.275 ppm。本研究采用猪寡核苷酸微阵列对基因表达水平进行检测。本研究报道了CYP2E1与CYP2A13的差异表达，并提示FMO1、HSD17B13、CBR4及多种转录因子参与相关过程。整体实验设计：本研究以肝脏为研究材料，采用实验室自制的双色猪寡核苷酸微阵列，对比25头高粪臭素水平公猪与25头低粪臭素水平公猪的基因表达差异。