RNA seq on tumors from two groups of mice myofibroblast-collagen1 knockout group and control group)

RNA sequencing analysis on tumor samples from two groups of mice (1) KPPF;Col1smaKO myfibroblast-collagen1 knockout group and (2) KPPF control group. Collagen1 is the most abundant extracellular matrix protein component in desmoplastic pancreatic tumors. In this study, we establish the transgenic mouse model KPPF;Col1smaKO mice with pancreatic tumors deleted for myofibroblast-derived collagen1. Then we use RNA-seq to examine the global changes of gene expression profile in tumors of KPPF;Col1smaKO mice (as compared to control KPPF mice). A total of 7 RNA samples from tumors from two groups of age-matched mice (1) KPPF;Col1smaKO myfibroblast-collagen1 knockout group, 4 mice; and (2) KPPF control group, 3 mice.

本研究针对两组小鼠的肿瘤样本开展RNA测序（RNA sequencing）分析：(1) KPPF;Col1smaKO 成肌纤维细胞源性胶原1敲除组（myfibroblast-collagen1 knockout group），(2) KPPF对照组。胶原1（Collagen1）是促结缔组织增生性胰腺癌（desmoplastic pancreatic tumors）中丰度最高的细胞外基质蛋白组分。本研究构建了成肌纤维细胞源性胶原1缺失的胰腺癌转基因小鼠模型KPPF;Col1smaKO，随后采用RNA-seq（RNA sequencing）技术，对比KPPF对照小鼠，分析该模型小鼠肿瘤组织的全基因表达谱全局变化。本研究共收集两组年龄匹配小鼠的肿瘤组织RNA样本共7份：其中KPPF;Col1smaKO成肌纤维细胞源性胶原1敲除组4只小鼠，对应样本4份；KPPF对照组3只小鼠，对应样本3份。