The NtrYX two-component system regulates the bacterial cell envelope

To investigate the R. sphaeroides NtrYX two component system on RNA transcript levels, we used global RNA sequencing (RNA-seq) to compare transcript abundance in parent and DntrYX strains grown under aerobic conditions. Our previous studies were performed in strains deficient at synthesizing the polyhydroxybutyrate, so we used strains D0382 and DNtrYXD0382, herein referred to as parent and DntrYX, respectively. We also used ChIP-seq to identify NtrX binding sites throughout the genome. Comparing ∆ntrYX to parent strain for RNA-seq and using ChIP-seq to map NtrX binding sites

为探究球形红杆菌（R. sphaeroides）NtrYX双组分系统对RNA转录水平的调控效应，本研究采用全局RNA测序（RNA-seq）技术，对比有氧培养条件下亲本菌株与ΔntrYX菌株的转录本丰度。此前本团队的相关研究均以聚羟基丁酸酯合成缺陷菌株为实验材料，因此本研究选用菌株D0382与DNtrYXD0382，下文分别将其称为亲本菌株与ΔntrYX菌株。此外，本研究还通过染色质免疫共沉淀测序（ChIP-seq）技术，在全基因组范围内鉴定NtrX的结合位点。本研究通过RNA-seq对比ΔntrYX菌株与亲本菌株的转录组差异，并借助ChIP-seq完成NtrX全基因组结合位点的定位分析。