Gene expression in B cells from mice with the autoimmune disease nephrotoxic nephritis

Studying the differences in glycosylation in B cells of normal mice and mice with an autoimmune disease (nephrotoxic nephritis). Differences in glycosylation in B cells of normal mice and mice with an autoimmune disease (nephrotoxic nephritis) were studied via gene expression analysis. RNA from mouse resting spleen B cells and B cells stimulated with antiCD40, CpG, and IL4 for 5 days, was isolated and prepared. One sample for each class was prepared. RNA was labeled and hybridized to the GLYCOv3 array. Resulting Gene expression patterns were analyzed.

本研究旨在探究正常小鼠与罹患自身免疫性疾病（肾毒性肾炎，nephrotoxic nephritis）的小鼠的B细胞糖基化差异，并通过基因表达分析手段完成了相关研究。我们分别从两类小鼠体内分离并制备了RNA样本：样本来源包括静息状态的脾脏B细胞，以及经抗CD40、CpG与IL4刺激培养5天的B细胞；每个实验类别均制备了一份样本。随后对RNA进行标记，将其与GLYCOv3芯片（GLYCOv3 array）进行杂交，最终对所得的基因表达模式进行了分析。