Transcriptome of red macroalgae Gracilaria lemaneiformis. Gracilariopsis lemaneiformis cultivar:981

This study aimed at systematically explaining how G. lemaneiformis respond to N deprivation.The G. lemaneiformis（strain 981 ） used in this study were obtained from Nan’ao Island cultivation field (116.5°E, 23.3°N), Shantou, Guangdong, China. G. lemaneiformis cultured grown in artificial sea water for 4 days before the onset of nitrogen deprived experiment with supplying nitrogen source. Continuously, the culture were distributed into two treatments. Lemaneiformis, N-replete treatment and N-deprived treatment, with the maintained light level and temperature at 21±1℃ for 20 days. During the experiment, We refresh the artificial seawater of two nitrogen treatments every two days. the transcriptomics were employed by Illuminia HiSeq 2000. The assembled transcripts contained 25937 Unigenes and 18329 were annotated and classified by Gene Ontology (GO).

本研究旨在系统阐释龙须菜（G. lemaneiformis）对氮缺乏的响应机制。本研究所用的981株龙须菜采自中国广东省汕头市南澳岛的养殖海域（东经116.5°，北纬23.3°）。实验前，将供试藻株置于添加氮源的人工海水中预培养4天，随后启动氮缺乏处理实验。将预培养后的培养体系分为两组，分别为氮充足组与氮缺乏组，实验期间维持温度为21±1℃，光照条件恒定，持续培养20天。实验过程中，每两日更换一次两组处理所用的人工海水。本研究采用Illumina HiSeq 2000平台开展转录组测序分析。最终组装得到的转录本集共包含25937个单基因（Unigene），其中18329个单基因通过基因本体论（Gene Ontology，GO）数据库完成注释与功能分类。