Direct effects of Methamphetamine and HIV-1 Tat in the recruitment of RNA Polymerase to innate immune cell gene promoters

Genome-wide maps of RNA polymerase II binding to chromatin in THP1 monocytic cell lines following Meth and HIV Tat exposure We report the most immediate effects of exposure to Methamphetamine and HIV Tat peptide, and their interactions, revealed by the recruitment of RNA Polymerase to gene promoters, in innate immune THP1 cells. Overall design: Examination of RNA Polymerase binding to chromatin in 1 cell line 15 minutes after the exposure to Methamphetamine or HIV Tat, or both.

本数据集涵盖甲基苯丙胺（Methamphetamine，缩写Meth）与人类免疫缺陷病毒Tat蛋白（HIV Tat）暴露后，THP1单核细胞系中RNA聚合酶II（RNA polymerase II）结合染色质的全基因组图谱。本研究报道了甲基苯丙胺与HIV Tat肽暴露及其交互作用所产生的最早期效应，该效应通过固有免疫THP1细胞内RNA聚合酶向基因启动子区域的募集得以揭示。实验设计概述：以THP1单核细胞系为研究对象，在分别暴露于甲基苯丙胺、HIV Tat蛋白，或二者联合暴露15分钟后，检测RNA聚合酶与染色质的结合情况。