CLEM of TRIM5a-YFP in HeLa cells

HeLa cell line 25 expressing stable TRIM5a-YFP. Correlated light electron microscopy. Concentration of 200 nm red fluorescent beads 1/300. 20 nm gold highly concentrated. Manual blotting with Whatman 40. Fibronectin coated with 0.25 mg/ml diluted with PBS

本数据集对应的样本为稳定表达TRIM5α-黄色荧光蛋白（YFP）的HeLa细胞系25；实验采用关联光电子显微术；使用粒径为200 nm的红色荧光微球，使用浓度为1/300，并辅以高浓度的20纳米金颗粒；实验过程中采用Whatman 40号滤纸进行手动印迹；以经磷酸缓冲盐溶液（PBS）稀释至0.25 mg/ml的纤连蛋白完成包被。