Illumina RNA-Seq, transcript models

Structural annotation/transcript models of RNA (cDNA) sequenced from six different tissues (brain, ileum, lung, ovary, spleen, testis) from the tufted duck on Illumina platform.Raw sequencing data was quality-checked with FastQC, adapters and contamination trimmed with Trimmomatic, quality-checked once again, mapped to the tufted duck reference genome with Hisat2, and transcript models assembled with StringTie2.Trimmomatic options: ${ILLUMINACLIP:TruSeq3-PE_rk.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36}Hisat2 options: ${--dta --rna-strandness RF}Stringtie2 options: ${--fr}

本数据集包含基于Illumina测序平台，从凤头潜鸭（tufted duck）的6种不同组织（脑、回肠、肺、卵巢、脾脏、睾丸）中提取的RNA（互补DNA，cDNA）的测序数据对应的结构注释与转录本模型。
原始测序数据先通过FastQC完成质量校验，随后使用Trimmomatic去除接头序列与污染序列，再次进行质量验证后，利用Hisat2将测序reads比对至凤头潜鸭参考基因组，最终通过StringTie2组装得到转录本模型。
Trimmomatic 参数配置：${ILLUMINACLIP:TruSeq3-PE_rk.fa:2:30:10 LEADING:3 TRAILING:3 SLIDINGWINDOW:4:15 MINLEN:36}
Hisat2 参数配置：${--dta --rna-strandness RF}
Stringtie2 参数配置：${--fr}