Insulin resistance, microbiota and fat distribution changes by a new model of vertical sleeve gastrectomy in obese rats. Rat gastrectomy healthy obese microbiota

Bariatric surgery is known to improve metabolic health, reducing insulin resistance and modulating the gut microbiota upon weight loss. We performed a novel vertical sleeve gastroectomy (GG) procedure in obese rats on a high fat diet enabling us to dissect diabetes from body weight. Together with improved insulin sensitivity, modified gut hormones, blood lipid profiles and a redistribution of fat storage in animals post GG compared to a sham operated (SO) group of obese rats, we observed significant differences within both the diversity and architecture of the intestinal microbiome. The GG animals had higher alpha and beta diversity compared to the SO group, and significant differences in relative abundance of different gut bacteria at the Class and Genus level, many previously linked to metabolic health both in rodent models and in humans

已知减重代谢手术（Bariatric surgery）可改善机体代谢健康，在减轻体重的同时缓解胰岛素抵抗并调节肠道微生物群。本研究针对高脂饮食喂养的肥胖大鼠，开发了一种新型袖状胃切除术（vertical sleeve gastroectomy, GG）术式，从而能够将糖尿病相关效应与体重变化分离开来。与假手术组（sham operated, SO）肥胖大鼠相比，GG组大鼠不仅胰岛素敏感性得到提升、肠道激素谱发生改变、血脂谱得以优化，且体内脂肪储存分布出现重分布；同时我们观察到两组大鼠肠道菌群的多样性与群落结构均存在显著差异：GG组大鼠的α多样性与β多样性均高于SO组，且在菌群纲（Class）与属（Genus）水平上，不同肠道细菌的相对丰度存在显著差异，其中诸多类群此前已在啮齿动物模型与人类研究中被证实与代谢健康密切相关。