Loss of BraA09g066480.3C leads to a wax-less phenotype in flowering Chinese cabbage. Loss of BraA09g066480.3C leads to a wax-less phenotype in flowering Chinese cabbage

PURPOSE:To clarify the mechanism of the wax deficiency, the wax-less flowering Chinese cabbage doubled-haploid (DH) line ‘CX001’ and Chinese cabbage DH line ‘FT’, obtained from isolated microspore culture, were used in the experiments. Transcriptome analysis indicated that BraA09g066480.3C was expressed in ‘FT’ but not in ‘CX001’.The work presented herein demonstrated that BraA09g066480.3C was the causal gene for wax-less flowering Chinese cabbage ‘CX001’ Overall design: Stems of ‘FT’ and ‘CX001’ were selected for RNA extraction, with three replicates per sample.

研究目的：为阐明蜡质缺失的分子机制，本实验采用通过游离小孢子培养获得的无蜡质开花菜薹双单倍体（doubled-haploid, DH）株系‘CX001’与白菜DH株系‘FT’作为实验材料。转录组分析结果显示，基因BraA09g066480.3C在‘FT’中存在表达，而在‘CX001’中未检测到表达。本研究证实，BraA09g066480.3C即为导致无蜡质开花菜薹‘CX001’表型的因果基因。总体实验设计：选取‘FT’与‘CX001’的茎部组织进行RNA提取，每个样本设置3次生物学重复。