wastewater metagenome Raw sequence reads

Sewage spills can release antibiotic resistant bacteria to surface waters, contributing to environmental reservoirs and potentially impacting human health. In this study, culturable VRE and/or vanA genes were detected in sediment and water samples after a sewage spill released more than 500,000 gallons of untreated sewage in a residential neighborhood. Illumina next generation sequencing (NGS) of environmental DNA from sediment and water revealed the temporal changes in the microbial community after a major influx of untreated sewage.

污水泄漏可将耐药菌释放至地表水体，促成耐药菌环境储库的形成，并可能对人类健康造成潜在威胁。本研究针对某居民区发生的单次泄漏量超50万加仑未经处理污水的事件，在其后续采集的沉积物与水样中检出了可培养耐万古霉素肠球菌（Vancomycin Resistant Enterococcus, VRE）及/或vanA基因。利用伊卢米纳（Illumina）下一代测序（NGS）技术对沉积物与水样中的环境DNA进行测序，揭示了未经处理污水大量涌入后微生物群落的时间动态变化。