Integrative transcriptomic analysis uncovers the miRNA-centric regulation of Japanese encephalitis virus infection in porcine trophoblast cells [miRNA-Seq]

To characterize porcine placental miRNAs, we performed small RNA-seq experiments using porcine placental tissues and the porcine placental trophoblast cell line pTr. To investigate the intracellular responses of pTr cells following JEV infection, RNA-seq and small RNA-seq experiments were conducted on pTr cells with or without JEV infection. Overall design: We collected placental samples from first - parity naturally delivered Duroc gilts, pTr cell samples under normal culture conditions, and pTr cell samples at 24 and 48 hours post - infection with JEV as well as non - infected samples. Two replicates per sample were used for RNA-seq and small RNA-seq experiments.

为解析猪胎盘微小RNA（miRNAs）的表达特征，本研究利用猪胎盘组织及猪胎盘滋养层细胞系pTr开展了小RNA测序（small RNA-seq）实验。为探究乙型脑炎病毒（JEV）感染pTr细胞后引发的细胞内应答反应，本研究对感染与未感染JEV的pTr细胞分别进行了RNA测序（RNA-seq）与小RNA测序实验。
整体实验设计：采集初产自然分娩杜洛克后备母猪的胎盘样本、正常培养条件下的pTr细胞样本，以及JEV感染后24小时、48小时的pTr细胞样本与未感染对照样本；所有样本均设置2个生物学重复，用于后续RNA-seq及small RNA-seq实验。