Rice met1-2 mutant callus multi omics sequencing data

CG methylation (mCG) is essential for preserving genome stability in mammals, but this link remains obscure in plants. OsMET1-2 is the major rice DNA methyltransferase, plays critical roles in maintaining mCG in rice. Null mutation of OsMET1-2 causes massive CG hypomethylation, rendering the mutant suitable to address the role of mCG in maintaining genome integrity in plants. Here, we sequenced the genome, methylome, and transcriptome of tissue cultures of OsMET1-2 homozygous (-/-) and heterozygous (+/-) mutants, and isogenic wild-type, for exploring the role of mCG on rice genome stability and TE mobility.

CG甲基化（mCG）对维持哺乳动物基因组稳定性至关重要，但该关联在植物中仍不甚明晰。OsMET1-2是水稻的主要DNA甲基转移酶，在维持水稻体内mCG水平方面发挥关键作用。OsMET1-2的纯合无效突变会引发大规模CG低甲基化，使得该突变体成为探究植物中mCG维持基因组完整性作用的理想实验材料。本研究对OsMET1-2纯合（-/-）、杂合（+/-）突变体及同基因背景野生型的组织培养样本开展了基因组、甲基化组与转录组测序，以此探究mCG在水稻基因组稳定性及转座因子（TE）移动性调控中的作用。