Raw sequence reads of whole transcriptome sequencing to identify prognostic markers in hypoxic mesothelioma. Homo sapiens

Malignant Mesothelioma (MM) is a type of cancer arising from the mesothelium, a thin layer of cells, lining internal organs. Therapeutic measures, which include chemo- and radiotherapy, and surgery in some cases, poorly increase life expectancy and there is no permanent cure for this disease. Therefore, early detection and prognostic prediction are important to improve the survival in MM patients. Pleural MM induces respiratory distress leading to hypoxia. Hypoxia, in turn, leads to accumulation of adenosine which favors tumor progression by suppressing T cell mediated immune response. Targeting adenosine receptors recently emerged as a promising anti-tumoral strategy. To mimic active hypoxia, mesothelioma cell line REN was treated with NECA [an adenosine receptor agonist] for 2 hours which effectively induced intracellular CREB phosphorylation. Therefore, we have performed whole transcriptomics analysis, we studied expression pattern of NECA-treated cells compared to control.

恶性胸膜间皮瘤（Malignant Mesothelioma, MM）是一类起源于间皮（mesothelium）的恶性肿瘤，间皮为内衬于内脏器官的薄层细胞结构。当前针对该疾病的治疗手段包括化学治疗、放射治疗，部分病例可联合外科手术，但此类方案仅能有限延长患者生存期，且目前尚无根治方法。因此，早期检测与预后预测对改善恶性胸膜间皮瘤患者的生存状况至关重要。胸膜间皮瘤可引发呼吸困难，进而导致缺氧；缺氧会进一步促使腺苷蓄积，通过抑制T细胞介导的免疫应答促进肿瘤进展。近年来，靶向腺苷受体已成为颇具前景的抗肿瘤策略。为模拟活跃缺氧状态，本研究使用NECA（一种腺苷受体激动剂）处理间皮瘤细胞系REN，孵育2小时后可有效诱导细胞内CREB磷酸化。基于此，本研究开展了全转录组学分析，对比了NECA处理组与对照组细胞的基因表达谱。