The Polyamine-Hypusine Circuit Controls an Oncogenic Translational Program Necessary for MYC-Driven Lymphomagenesis [Ribo-seq]

Hypusinated EiF5A likely impacts diverse cellular pathways through direct regulation of its translational targets or via secondary effects on other biological processes. We examined the global effects of knockdown of EiF5A and Dhps on the transcriptome and translational landscape of MYC-induced lymphoma. Overall design: To evaluate the effects of EiF5A or Dhps depletion on translation, Ribosome profiling analyses of Em-Myc; Rosa26-rtTA2 lymphoma cells that inducibly express sh-Ren, sh-EiF5A or sh-Dhps were performed along with RNA-seq.

羟腐胺赖氨酸修饰的真核翻译起始因子5A（Hypusinated EiF5A）或可通过直接调控其翻译靶标，或经由对其他生物学过程的次级效应，影响多样化的细胞通路。我们检测了敲低EiF5A与脱氧hypusine合酶（Dhps）对MYC诱导的淋巴瘤细胞转录组与翻译组谱的全局调控效应。实验设计概述：为评估EiF5A或Dhps敲低对翻译过程的影响，本研究对可诱导表达sh-Ren、sh-EiF5A或sh-Dhps的Em-Myc; Rosa26-rtTA2淋巴瘤细胞开展了核糖体谱分析（Ribosome profiling）与RNA测序（RNA-seq）的联合检测。