Quantitative eDNA metabarcoding survey at the Hebisawagawa front reservoir. Actinopterygii environmental sample

The survey sites were located in the Hebisawagawa front reservoir at the Miharu Reservoir in Fukushima Prefecture, Japan. The total area of the Hebisawagawa front reservoir was about 44,000 m2. This front reservoir is connected to the main reservoir by a channel with a width of 5 m and a depth of 5 m. It is isolated during the summer season (June 11th to October 10th) when the water level is low. Water samples were collected at three sites once a week from March 26 to August 13, 2019, and March 3 to August 25, 2020. Quantitative metabarcoding approach was used to simultaneously quantify the eDNA concentration of multiple fish species.

本研究的采样点设于日本福岛县三春水库（Miharu Reservoir）下辖的蛇沢川前置水库（Hebisawagawa front reservoir）。该前置水库总面积约44000平方米，通过宽5米、深5米的渠道与主水库相连；在夏季（6月11日至10月10日）水位较低时，该前置水库会与主水库相互隔绝。研究分别于2019年3月26日至8月13日、2020年3月3日至8月25日期间，每周在3个采样点采集水样。本研究采用定量元条形码（quantitative metabarcoding）技术，同步测定多种鱼类的环境DNA（environmental DNA）浓度。