Regional Differences in gene expression and promoter usage in aged human brains. Homo sapiens

Cap analysis of gene expression (CAGE) and massive parallel sequencing were used to profile the promoterome of aged human brains from five regions, namely: caudate, frontal cortex, hippocampus, putamen and temporal cortex. Overall design: 25 RNA libraries from post-mortem brain tissue (five caudate, five frontal, 5 hippocampus, 5 putamen, five temporal RNA libraries from seven individuals) were processed using CAGE protocol and CAGE tags derived from the 25 libraries were sequenced with Illumina.

本研究采用基因表达帽分析（Cap Analysis of Gene Expression, CAGE）与大规模并行测序技术，对5个脑区的衰老人类大脑启动子组进行表征分析，所涉脑区包括尾状核、额叶皮层、海马体、壳核与颞叶皮层。总体实验设计：从7名个体的死后脑组织中获取25个RNA文库，各脑区文库数量分别为尾状核5个、额叶皮层5个、海马体5个、壳核5个、颞叶皮层5个；采用CAGE实验流程对上述文库进行处理，并使用Illumina测序平台对来自25个文库的CAGE标签进行测序。