Comprehensive analysis of the SWR1 complex reveals the coupling of H2A.Z deposition with nucleosome sliding in Arabidopsis [ChIP-Seq]

We identified three classes of previously uncharacterized plant-specific SWR1 components: MBD9, TRA1a/1b and CHR11/17. We found that CHR11/17 were recruited to the SWR1 complex and facilitate the coupling of H2A.Z deposition with nucleosome sliding, thereby co-regulating gene expression, development, and flowering time. Overall design: protein-DNA compelxes were extracted by antibody, and DNA were finally extracted and sequenced.

本研究鉴定出三类此前未被表征的植物特异性SWR1复合物组分：MBD9、TRA1a/1b与CHR11/17。研究发现，CHR11/17可被招募至SWR1复合物中，介导H2A.Z沉积与核小体滑动的偶联过程，进而协同调控基因表达、植物发育及开花时间。实验整体设计：通过抗体富集提取蛋白质-DNA复合物，最终提取DNA并进行测序。