one-to-one orthologs

Manakins represent a family of small suboscine passerine birds characterized by their elaborate courtship displays, non-monogamous mating system, and sexual dimorphism. This family thus serves as a good model for the study of sexual selection. Here we present the genome assemblies of four manakin species (<em>Cryptopipo holochlora, Dixiphia pipra, Machaeropterus deliciosus</em> and <em>Masius chrysopterus</em>) generated by Single-tube Long Fragment Read (stLFR) technology. The assembled genome sizes ranged from 1.10 Gb to 1.19 Gb, with average scaffold N50 of 28 Mb and contig N50 of 169 Kb. On average, 9.82% genome region was annotated as repetitive elements, and 12,059 protein-coding genes were annotated in the genomes. We further identified about 75 Mb Z-linked sequences in manakins, containing 585 to 751 genes, and 600 Kb pseudoautosomal region (PAR). One interesting finding of these Z-linked sequences is that Z-to-autosome/PAR reversal could happen in <em>Masius chrysopterus</em>. These manakin genomes will contribute to a deeper understanding of evolutionary history and sexual selection in these species.

娇鹟科是一类小型亚鸣禽雀形目鸟类，以精致的求偶炫耀行为、非单配制交配体系与性别二态性为典型特征，因此成为性选择研究的优良模型类群。本研究采用单管长片段读长技术（Single-tube Long Fragment Read, stLFR），完成了4种娇鹟的基因组组装，涵盖物种包括Cryptopipo holochlora、Dixiphia pipra、Machaeropterus deliciosus及Masius chrysopterus。组装后的基因组大小介于1.10 Gb至1.19 Gb之间，平均支架N50（scaffold N50）为28 Mb，平均重叠群N50（contig N50）为169 Kb。经注释，平均9.82%的基因组区域为重复序列元件，各基因组中共注释得到12059个蛋白质编码基因。此外，本研究还在娇鹟基因组中鉴定出约75 Mb的Z连锁序列，包含585至751个基因，以及600 Kb的伪常染色体区域（pseudoautosomal region, PAR）；其中一项有趣的发现是，Masius chrysopterus中存在Z染色体-常染色体/PAR反转现象。上述娇鹟基因组数据将为深入解析该类群的进化历史与性选择机制提供重要支撑。