Genome-wide map of vitamin D receptor (VDR) binding in THP-1 cells. Homo sapiens

Analysis of acute effects of ligand-treatment on vitamin D receptor binding genome-wide using ChIP-seq. THP-1 monocytic leucemia cells were treated with 1?,25(OH)2D3 (1,25D) or left unstimulated to investigate the acute effects of VDR chromatin occupancy. We identified in total 2340 VDR binding sites with and without the ligand. Without the ligand, there is a considerable presence of VDR already on the chromatin. However, upon a short (40 min) ligand treatment VDR shifts from sites that rarely contain a DR3 type element to sites that frequently contain one or more DR3-type element. Overall design: Genome-wide identification of VDR binding in THP-1 cells at the unstimulated state and after 40 min ligand (10 nM 1?,25(OH)2D3 (1,25D, calcitriol)) treatment.

本研究借助染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing, ChIP-seq）技术，分析配体处理对全基因组范围内维生素D受体（vitamin D receptor, VDR）结合事件的急性影响。本研究以THP-1单核细胞白血病细胞为实验模型，分别采用1α,25(OH)₂D₃（1,25D）处理细胞，同时设置未刺激对照组，以探究VDR染色质占位的急性变化效应。本研究共鉴定出2340个在有配体和无配体条件下的VDR结合位点。在未添加配体的状态下，染色质上已存在大量VDR结合位点；然而经40分钟短时长配体处理后，VDR的结合位点从极少包含DR3型元件的基因组区域，转移至频繁含有一个或多个DR3型元件的区域。实验设计：分别在未刺激状态，以及经10 nM 1α,25(OH)₂D₃（1,25D，骨化三醇）处理40分钟后，对THP-1细胞开展全基因组范围的VDR结合位点鉴定。