RNAseq analysis of the human neutrophil transcriptome, with and without in vitro cytokine stimulation

We report cytokine specific changes in gene expression in the human neutrophil transcriptome using TNF-alpha and GM-CSF stimulation of healthy neutrophils Healthy human neutrophils were stimulated with TNF-alpha or GM-CSF for 1h in vitro. RNA was analysed by SOLiD and Illumina sequencing. RNA from one biological donor was sequenced on both platforms, and two different biological donors were sequenced by Illumina.

本研究报道了健康人中性粒细胞经肿瘤坏死因子-α（TNF-alpha）与粒细胞-巨噬细胞集落刺激因子（GM-CSF）体外刺激后，其转录组中细胞因子特异性基因的表达变化。实验中，健康人中性粒细胞于体外经TNF-alpha或GM-CSF刺激1小时，随后通过SOLiD与Illumina测序技术对总RNA进行分析。其中，1名健康供体的RNA在两个测序平台均完成测序，另有2名不同健康供体的RNA仅通过Illumina平台完成测序。