Mouse embryonic RNA-seq. Mouse embryonic RNA-seq

The study was aimed at interrogating the early stages of blood cell development within the embryo. Blood develops from mesoderm, which is specified at gastrulation. We therefore sorted cells from mouse embryos between E6.5 and E7.75 to capture gastrulation and the emergence of mesoderm, followed by specificiation of the first mature blood cells.

本研究旨在解析胚胎内血细胞发育的早期阶段。血液由中胚层（mesoderm）发育而来，中胚层于原肠胚形成（gastrulation）阶段被特化确立。因此，我们对发育时段介于E6.5至E7.75的小鼠胚胎细胞进行分选，以捕获原肠胚形成与中胚层出现的关键过程，进而捕捉首批成熟血细胞的特化事件。